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plcko vector  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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    Structured Review

    New England Biolabs plcko vector
    Plcko Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plcko+plcko+vector/BsmBI-v2/bio_rxiv__2025__04__14__648628-154-1-10
    Average 99 stars, based on 1648 article reviews
    plcko vector - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cloning:

    Article Title: Frizzled5 protein-binding agents
    Article Snippet: Cloning of Individual gRNA Target Sequences into pLCKO: pLCKO vector was digested with BfuAI (NEB) and gel purified with PureLink combo kit (ThermoFisher #K2200-01). .. Cloning of Individual gRNA Target Sequences into pLCKO: pLCKO vector was digested with BfuAI (NEB) and gel purified with PureLink combo kit (ThermoFisher #K2200-01). .. Oligonucleotides were first phosphorylated with PNK (ThermoFisher #AM2310) and annealed through 95 degrees centigrade incubation for 10 minutes followed by slope ramp-down to room temperature.

    Plasmid Preparation:

    Article Title: Frizzled5 protein-binding agents
    Article Snippet: Cloning of Individual gRNA Target Sequences into pLCKO: pLCKO vector was digested with BfuAI (NEB) and gel purified with PureLink combo kit (ThermoFisher #K2200-01). .. Cloning of Individual gRNA Target Sequences into pLCKO: pLCKO vector was digested with BfuAI (NEB) and gel purified with PureLink combo kit (ThermoFisher #K2200-01). .. Oligonucleotides were first phosphorylated with PNK (ThermoFisher #AM2310) and annealed through 95 degrees centigrade incubation for 10 minutes followed by slope ramp-down to room temperature.

    Purification:

    Article Title: Frizzled5 protein-binding agents
    Article Snippet: Cloning of Individual gRNA Target Sequences into pLCKO: pLCKO vector was digested with BfuAI (NEB) and gel purified with PureLink combo kit (ThermoFisher #K2200-01). .. Cloning of Individual gRNA Target Sequences into pLCKO: pLCKO vector was digested with BfuAI (NEB) and gel purified with PureLink combo kit (ThermoFisher #K2200-01). .. Oligonucleotides were first phosphorylated with PNK (ThermoFisher #AM2310) and annealed through 95 degrees centigrade incubation for 10 minutes followed by slope ramp-down to room temperature.



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